Pathogen Detection Tool Could Change Infectious Diseases Diagnoses.

Scientists at the University of Utah, ARUP Laboratories, and IDbyDNA, Inc., have developed ultra-fast, meta-genomics analysis software called Taxonomer that dramatically improves the accuracy and speed of pathogen detection. In a paper published today in Genome Biology, the collaborators demonstrated the ability of Taxonomer to analyze the sequences of all nucleic acids in a clinical specimen (DNA and RNA) and to detect pathogens, as well as profile the patient's gene expression, in a matter of minutes. Infectious diseases are one of the biggest killers in the world. Almost 5 million children under age 5 die each year from infectious diseases worldwide, yet many infections are treatable if the pathogen culprit can be quickly and accurately identified. "In the realm of infectious diseases, this type of technology could be as significant as sequencing the human genome," says co-author Mark Yandell, PhD, professor of human genetics at the University of Utah (U of U), H.A. & Edna Benning Presidential Endowed Chair holder, co-director of the USTAR Center for Genetic Discovery, and co-founder of IDbyDNA. "Very few people have inherited genetic disease. But at some point, everyone gets sick from infections." It is difficult for infectious pathogens to hide when their genetic material is laid bare. Taxonomer opens up an entirely new approach for infectious disease diagnosis, driven by sophisticated genomic analysis and computational technologies. After a patient's sample is sequenced, the data are uploaded via the internet to Taxonomer. In less than one minute, the tool displays a thumbnail inventory of all pathogens in the sample, including viruses, bacteria, and fungi. The interactive, real-time user interface of Taxonomer is powered by the IOBIO system developed by the laboratory of Gabor Marth, DSc, professor of human genetics at the U of U and co-Director of the USTAR Center for Genetic Discovery. Schlaberg explains that Taxonomer can identify an infection without the physician having to decide what to test for, something a PCR-based test cannot do. In other words, a doctor doesn't have to suspect the cause of a patient's infection, but can instead simply ask, "What does my patient have?" and Taxonomer will identify the pathogens. In the new study, Taxonomer was put to the test with real-world cases using data published by others and samples provided by ARUP Laboratories and the Centers for Disease Control and Prevention (CDC). Taxonomer determined that some patients who exhibited Ebola-like symptoms in the recent African outbreak did not have Ebola but severe bacterial infections that likely caused their symptoms. "This technology can be applied whenever we don't know the cause of the disease, including the detection of sudden outbreaks of disease. It is very clear we urgently need more accurate diagnostics to greatly enhance the ability of public health response and clinical care," says Seema Jain, MD, medical epidemiologist at the CDC. Another unique feature of Taxonomer is its ability to delve into human gene expression profiling, which provides information on how or if the patient's body is reacting to an infection. "As a clinician, this gives you a better idea, when we identify a pathogen whether it is really the cause of the disease," says Carrie L. Byington, MD, professor of pediatrics of the U of U and co-director of the Center for Clinical and Translational Science. The tool will also show if the patient is responding to a bacterial or viral infection when we don't find a pathogen or when we find multiple potential causes.She states that she sees the exceptional value of this tool for treating children, who experience more life-threatening infections early in life. "Seeing how a host [patient] reacts is extremely valuable; I believe this is a paradigm shift in how we diagnose people. culled from R&D Magazine.

COMMON REASONS FOR VACCINATION FAILURE.

The success of the venture depends on following strict health protocols that are wrapped around simple but effective bio security strategy. The health protocol involves vaccinations coupled with medications when needed. Vaccination refers to a method of disease prevention in healthy animals by challenging the animal with an attenuated/killed agent of a disease so as to elicit the production of antibodies. When vaccinations are done properly and immunity conferred,the animal is said to have been immunized against such causative agents of disease.Vaccination though stimulates antibodies production to protect the animals ,but if not done properly ;the desired immunity will not be conferred.When immunity is not conferred,2 major causes are fingered.These are vaccine failure or vaccine break. When immunity is not conferred due to vaccine break,the following are causes; 1)when the cold chain is broken; usually from point of sale to area you need them.The vaccines are to be transported in coolers with enough ice packs for the trip.The potency of the vaccines cant be guaranteed when this occurs,and when used the required immunity is not conferred. 2) when using inappropriate dilution and concentration; this nullifies any effect the vaccine should have on the birds. 3)when expired vaccines are used,definitely no immunity can be conferred. While vaccine failure could occur in any of the following scenarios;1)using inappropriate dose; under/overdosing will not elicit any antibody reaction. 2)wrong dilution/route. 3)vaccination of sick birds. 4)stress on birds during handling especially in hot weather. 5)Use of chemicals along side the vaccines especially water purification solutions. 6)vaccinating birds when signs of diseases are visible in flock. Vaccinations though very essential, if not properly carried out will just be a waste of resources. When planning to vaccinate keep it simple,follow these guidelines; 1)place vaccine in ice pack. 2)engage qualified personnel. 3) use vaccine as directed by manufacturers.